8BER

Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) with T=3 topology

8BER の概要

• エントリーDOI: 10.2210/pdb8ber/pdb
• EMDBエントリー: 16009
• 分子名称: Core protein,Matrix protein 2,External core antigen (1 entity in total)
• 機能のキーワード: hbc, m2e, vlp, virus like particle
• 由来する生物種: Hepatitis B virus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 92878.99

構造登録者

Egorov, V.V.,Shvetsov, A.V.,Pichkur, E.B.,Shaldzhyan, A.A.,Zabrodskaya, Y.A.,Vinogradova, D.S.,Nekrasov, P.A.,Gorshkov, A.N.,Garmay, Y.P.,Kovaleva, A.A.,Stepanova, L.A.,Tsybalova, L.M.,Shtam, T.A.,Myasnikov, A.G.,Konevega, A.L. (登録日: 2022-10-21, 公開日: 2022-12-28, 最終更新日: 2024-11-13)

主引用文献

Egorov, V.V.,Shvetsov, A.V.,Pichkur, E.B.,Shaldzhyan, A.A.,Zabrodskaya, Y.A.,Vinogradova, D.S.,Nekrasov, P.A.,Gorshkov, A.N.,Garmay, Y.P.,Kovaleva, A.A.,Stepanova, L.A.,Tsybalova, L.M.,Shtam, T.A.,Myasnikov, A.G.,Konevega, A.L.
Inside and outside of virus-like particles HBc and HBc/4M2e: A comprehensive study of the structure.
Biophys.Chem., 293:106943-106943, 2022

PubMed Abstract: Hepatitis B virus core antigen (HBc) with the insertion of four external domains of the influenza A M2 protein (HBc/4M2e) form virus-like particles whose structure was studied using a combination of molecular modeling and cryo-electron microscopy (cryo-EM). It was also shown that self-assembling of the particles occurs inside bacterial cells, but despite the big inner volume of the core shell particle, purified HBc/4M2e contain an insignificant amount of bacterial proteins. It was shown that a fragment of the M2e corresponding to 4M2e insertion is prone to formation of amyloid-like fibrils. However, as the part of the immunodominant loop, M2e insertion does not show a tendency to intermolecular interaction. A full-atomic HBc-4M2e model with the resolution of about 3 Å (3.13 Å for particles of Т = 4 symmetry, 3.7 Å for particles of Т = 3 symmetry) was obtained by molecular modeling methods based on cryo-EM data.

PubMed: 36495688
DOI: 10.1016/j.bpc.2022.106943

実験手法

ELECTRON MICROSCOPY (4 Å)