8BEI

Structure of hexameric subcomplexes (Truncation Delta2-6) of the fractal citrate synthase from Synechococcus elongatus PCC7942

8BEI の概要

• エントリーDOI: 10.2210/pdb8bei/pdb
• EMDBエントリー: 16004
• 分子名称: Citrate synthase (1 entity in total)
• 機能のキーワード: fractal complex, transferase
• 由来する生物種: Synechococcus elongatus PCC 7942 = FACHB-805
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 263279.20

構造登録者

Lo, Y.K.,Bohn, S.,Sendker, F.L.,Schuller, J.M.,Hochberg, G. (登録日: 2022-10-21, 公開日: 2024-02-28, 最終更新日: 2024-09-11)

主引用文献

Sendker, F.L.,Lo, Y.K.,Heimerl, T.,Bohn, S.,Persson, L.J.,Mais, C.N.,Sadowska, W.,Paczia, N.,Nussbaum, E.,Del Carmen Sanchez Olmos, M.,Forchhammer, K.,Schindler, D.,Erb, T.J.,Benesch, J.L.P.,Marklund, E.G.,Bange, G.,Schuller, J.M.,Hochberg, G.K.A.
Emergence of fractal geometries in the evolution of a metabolic enzyme.
Nature, 628:894-900, 2024

PubMed Abstract: Fractals are patterns that are self-similar across multiple length-scales. Macroscopic fractals are common in nature; however, so far, molecular assembly into fractals is restricted to synthetic systems. Here we report the discovery of a natural protein, citrate synthase from the cyanobacterium Synechococcus elongatus, which self-assembles into Sierpiński triangles. Using cryo-electron microscopy, we reveal how the fractal assembles from a hexameric building block. Although different stimuli modulate the formation of fractal complexes and these complexes can regulate the enzymatic activity of citrate synthase in vitro, the fractal may not serve a physiological function in vivo. We use ancestral sequence reconstruction to retrace how the citrate synthase fractal evolved from non-fractal precursors, and the results suggest it may have emerged as a harmless evolutionary accident. Our findings expand the space of possible protein complexes and demonstrate that intricate and regulatable assemblies can evolve in a single substitution.

PubMed: 38600380
DOI: 10.1038/s41586-024-07287-2

実験手法

ELECTRON MICROSCOPY (3.06 Å)