8BDB

Ribulose-1,5-bisphosphate carboxylase/oxygenase from Griffithsia monilis

8BDB の概要

• エントリーDOI: 10.2210/pdb8bdb/pdb
• 分子名称: Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain, MAGNESIUM ION, ... (8 entities in total)
• 機能のキーワード: carbon dioxide fixation, photorespiration, lyase (carbon-carbon), photosynthesis
• 由来する生物種: Griffithsia monilis; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 571318.61

構造登録者

Andersson, I.,Gunn, L.H. (登録日: 2022-10-19, 公開日: 2023-06-14, 最終更新日: 2024-02-07)

主引用文献

Zhou, Y.,Gunn, L.H.,Birch, R.,Andersson, I.,Whitney, S.M.
Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.
Nat.Plants, 9:978-986, 2023

PubMed Abstract: Improving the carboxylation properties of Rubisco has primarily arisen from unforeseen amino acid substitutions remote from the catalytic site. The unpredictability has frustrated rational design efforts to enhance plant Rubisco towards the prized growth-enhancing carboxylation properties of red algae Griffithsia monilis GmRubisco. To address this, we determined the crystal structure of GmRubisco to 1.7 Å. Three structurally divergent domains were identified relative to the red-type bacterial Rhodobacter sphaeroides RsRubisco that, unlike GmRubisco, are expressed in Escherichia coli and plants. Kinetic comparison of 11 RsRubisco chimaeras revealed that incorporating C329A and A332V substitutions from GmRubisco Loop 6 (corresponding to plant residues 328 and 331) into RsRubisco increased the carboxylation rate (k) by 60%, the carboxylation efficiency in air by 22% and the CO/O specificity (S) by 7%. Plastome transformation of this RsRubisco Loop 6 mutant into tobacco enhanced photosynthesis and growth up to twofold over tobacco producing wild-type RsRubisco. Our findings demonstrate the utility of RsRubisco for the identification and in planta testing of amino acid grafts from algal Rubisco that can enhance the enzyme's carboxylase potential.

PubMed: 37291398
DOI: 10.1038/s41477-023-01436-7

実験手法

X-RAY DIFFRACTION (1.7 Å)