8BAW

X-ray structure of the CeuE Homologue from Geobacillus stearothermophilus - 5-LICAM siderophore analogue complex.

8BAW の概要

• エントリーDOI: 10.2210/pdb8baw/pdb
• 分子名称: Siderophore ABC transporter substrate-binding protein, FE (III) ION, N,N'-pentane-1,5-diylbis(2,3-dihydroxybenzamide), ... (4 entities in total)
• 機能のキーワード: periplasmic, siderophore binding protein, bacterial, siderophore analogue complex., 5-licam, metal transport
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67632.33

構造登録者

Blagova, E.V.,Miller, A.,Booth, R.,Dodson, E.J.,Duhme-Klair, A.K.,Wilson, K.S. (登録日: 2022-10-12, 公開日: 2023-07-12, 最終更新日: 2024-02-07)

主引用文献

Blagova, E.V.,Miller, A.H.,Bennett, M.,Booth, R.L.,Dodson, E.J.,Duhme-Klair, A.K.,Wilson, K.S.
Thermostable homologues of the periplasmic siderophore-binding protein CeuE from Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius.
Acta Crystallogr D Struct Biol, 79:694-705, 2023

PubMed Abstract: Siderophore-binding proteins from two thermophilic bacteria, Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius, were identified from a search of sequence databases, cloned and overexpressed. They are homologues of the well characterized protein CjCeuE from Campylobacter jejuni. The iron-binding histidine and tyrosine residues are conserved in both thermophiles. Crystal structures were determined of the apo proteins and of their complexes with iron(III)-azotochelin and its analogue iron(III)-5-LICAM. The thermostability of both homologues was shown to be about 20°C higher than that of CjCeuE. Similarly, the tolerance of the homologues to the organic solvent dimethylformamide (DMF) was enhanced, as reflected by the respective binding constants for these ligands measured in aqueous buffer at pH 7.5 in the absence and presence of 10% and 20% DMF. Consequently, these thermophilic homologues offer advantages in the development of artificial metalloenzymes using the CeuE family.

PubMed: 37428843
DOI: 10.1107/S2059798323004473

実験手法

X-RAY DIFFRACTION (1.471 Å)