8B9F

Structure of Echovirus 11 complexed with DAF (CD55) calculated from symmetry expansion

8B9F の概要

• エントリーDOI: 10.2210/pdb8b9f/pdb
• EMDBエントリー: 15930
• 分子名称: Complement decay-accelerating factor, Genome polyprotein, SPHINGOSINE, ... (5 entities in total)
• 機能のキーワード: echovirus, receptor, cd55, daf, virus
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 117270.91

構造登録者

Stuart, D.I.,Ren, J.,Qin, L.,Zhou, D. (登録日: 2022-10-05, 公開日: 2022-12-07, 最終更新日: 2024-10-16)

主引用文献

Zhou, D.,Qin, L.,Duyvesteyn, H.M.E.,Zhao, Y.,Lin, T.Y.,Fry, E.E.,Ren, J.,Huang, K.A.,Stuart, D.I.
Switching of Receptor Binding Poses between Closely Related Enteroviruses.
Viruses, 14:-, 2022

PubMed Abstract: Echoviruses, for which there are currently no approved vaccines or drugs, are responsible for a range of human diseases, for example echovirus 11 (E11) is a major cause of serious neonatal morbidity and mortality. Decay-accelerating factor (DAF, also known as CD55) is an attachment receptor for E11. Here, we report the structure of the complex of E11 and the full-length ectodomain of DAF (short consensus repeats, SCRs, 1-4) at 3.1 Å determined by cryo-electron microscopy (cryo-EM). SCRs 3 and 4 of DAF interact with E11 at the southern rim of the canyon via the VP2 EF and VP3 BC loops. We also observe an unexpected interaction between the N-linked glycan (residue 95 of DAF) and the VP2 BC loop of E11. DAF is a receptor for at least 20 enteroviruses and we classify its binding patterns from reported DAF/virus complexes into two distinct positions and orientations, named as E6 and E11 poses. Whilst 60 DAF molecules can attach to the virion in the E6 pose, no more than 30 can attach to E11 due to steric restrictions. Analysis of the distinct modes of interaction and structure and sequence-based phylogenies suggests that the two modes evolved independently, with the E6 mode likely found earlier.

PubMed: 36560629
DOI: 10.3390/v14122625

実験手法

ELECTRON MICROSCOPY (3.93 Å)