8B8S

Solution structure of tandem RRM1 and RRM2 domains of yeast NPL3

8B8S の概要

• エントリーDOI: 10.2210/pdb8b8s/pdb
• NMR情報: BMRB: 34761
• 分子名称: Serine/arginine (SR)-type shuttling mRNA binding protein NPL3 (1 entity in total)
• 機能のキーワード: serine/arginine (sr)-type mrna binding protein, paramagnetic relaxation enhancement (pre) based solution nmr structure, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18228.41

構造登録者

Kachariya, N.,Sattler, M.,Keil, P.,Strasser, K. (登録日: 2022-10-04, 公開日: 2022-11-09, 最終更新日: 2024-06-19)

主引用文献

Keil, P.,Wulf, A.,Kachariya, N.,Reuscher, S.,Huhn, K.,Silbern, I.,Altmuller, J.,Keller, M.,Stehle, R.,Zarnack, K.,Sattler, M.,Urlaub, H.,Strasser, K.
Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA.
Nucleic Acids Res., 51:831-851, 2023

PubMed Abstract: RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein-RNA and protein-protein interactions. Their roles have largely been analyzed by crude mutations, which abrogate multiple functions at once and likely impact the structural integrity of the large ribonucleoprotein particles (RNPs) these proteins function in. Using UV-induced RNA-protein crosslinking of entire cells, protein complex purification and mass spectrometric analysis, we identified >100 in vivo RNA crosslinks in 16 nuclear mRNP components in Saccharomyces cerevisiae. For functional analysis, we chose Npl3, which displayed crosslinks in its two RNA recognition motifs (RRMs) and in the connecting flexible linker region. Both RRM domains and the linker uniquely contribute to RNA recognition as revealed by NMR and structural analyses. Interestingly, mutations in these regions cause different phenotypes, indicating distinct functions of the different RNA-binding domains. Notably, an npl3-Linker mutation strongly impairs recruitment of several mRNP components to chromatin and incorporation of other mRNP components into nuclear mRNPs, establishing a so far unknown function of Npl3 in nuclear mRNP assembly. Taken together, our integrative analysis uncovers a specific function of the RNA-binding activity of the nuclear mRNP component Npl3. This approach can be readily applied to RBPs in any RNA metabolic process.

PubMed: 36583366
DOI: 10.1093/nar/gkac1206

実験手法

SOLUTION NMR
SOLUTION SCATTERING