8B7G

Structure of oxygen-degraded rsCherry

8B7G の概要

• エントリーDOI: 10.2210/pdb8b7g/pdb
• 関連するPDBエントリー: 8B65
• 分子名称: rsCherry, GLYCEROL, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: rscherry, red fluorescent protein, chemical modifications, fluorescent protein
• 由来する生物種: Discosoma sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31272.02

構造登録者

Bui, T.Y.H.,Van Meervelt, L. (登録日: 2022-09-29, 公開日: 2023-04-05, 最終更新日: 2024-02-07)

主引用文献

Bui, T.Y.H.,De Zitter, E.,Moeyaert, B.,Pecqueur, L.,Srinivasu, B.Y.,Economou, A.,Fontecave, M.,Van Meervelt, L.,Dedecker, P.,Pedre, B.
Oxygen-induced chromophore degradation in the photoswitchable red fluorescent protein rsCherry.
Int.J.Biol.Macromol., 239:124179-124179, 2023

PubMed Abstract: Reversibly switchable monomeric Cherry (rsCherry) is a photoswitchable variant of the red fluorescent protein mCherry. We report that this protein gradually and irreversibly loses its red fluorescence in the dark over a period of months at 4 °C and a few days at 37 °C. We also find that its ancestor, mCherry, undergoes a similar fluorescence loss but at a slower rate. X-ray crystallography and mass spectrometry reveal that this is caused by the cleavage of the p-hydroxyphenyl ring from the chromophore and the formation of two novel types of cyclic structures at the remaining chromophore moiety. Overall, our work sheds light on a new process occurring within fluorescent proteins, further adding to the chemical diversity and versatility of these molecules.

PubMed: 36972828
DOI: 10.1016/j.ijbiomac.2023.124179

実験手法

X-RAY DIFFRACTION (1.3 Å)