8B6P

X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 154-156 (cpHaloTag7_154-156)

Summary for 8B6P

• Entry DOI: 10.2210/pdb8b6p/pdb
• Related: 8B6N 8B6O 8B6Q
• Descriptor: Haloalkane dehalogenase, CHLORIDE ION (3 entities in total)
• Functional Keywords: haloalkane dehalogenase, halotag, halotag7, self-labeling protein, hydrolase
• Biological source: Rhodococcus sp.; More
• Total number of polymer chains: 2
• Total formula weight: 68616.78

Authors

Tarnawski, M.,Johnsson, K.,Hiblot, J. (deposition date: 2022-09-27, release date: 2023-10-11, Last modification date: 2024-03-06)

Primary citation

Huppertz, M.C.,Wilhelm, J.,Grenier, V.,Schneider, M.W.,Falt, T.,Porzberg, N.,Hausmann, D.,Hoffmann, D.C.,Hai, L.,Tarnawski, M.,Pino, G.,Slanchev, K.,Kolb, I.,Acuna, C.,Fenk, L.M.,Baier, H.,Hiblot, J.,Johnsson, K.
Recording physiological history of cells with chemical labeling.
Science, 383:890-897, 2024

PubMed Abstract: Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.

PubMed: 38386755
DOI: 10.1126/science.adg0812

Experimental method

X-RAY DIFFRACTION (1.1 Å)