8B6P

X-ray structure of the haloalkane dehalogenase HaloTag7 circular permutated at positions 154-156 (cpHaloTag7_154-156)

8B6P の概要

• エントリーDOI: 10.2210/pdb8b6p/pdb
• 関連するPDBエントリー: 8B6N 8B6O 8B6Q
• 分子名称: Haloalkane dehalogenase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: haloalkane dehalogenase, halotag, halotag7, self-labeling protein, hydrolase
• 由来する生物種: Rhodococcus sp.; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68616.78

構造登録者

Tarnawski, M.,Johnsson, K.,Hiblot, J. (登録日: 2022-09-27, 公開日: 2023-10-11, 最終更新日: 2024-03-06)

主引用文献

Huppertz, M.C.,Wilhelm, J.,Grenier, V.,Schneider, M.W.,Falt, T.,Porzberg, N.,Hausmann, D.,Hoffmann, D.C.,Hai, L.,Tarnawski, M.,Pino, G.,Slanchev, K.,Kolb, I.,Acuna, C.,Fenk, L.M.,Baier, H.,Hiblot, J.,Johnsson, K.
Recording physiological history of cells with chemical labeling.
Science, 383:890-897, 2024

PubMed Abstract: Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.

PubMed: 38386755
DOI: 10.1126/science.adg0812

実験手法

X-RAY DIFFRACTION (1.1 Å)