8B5P

Ternary structure of 14-3-3s, ERRg phosphopeptide and dual-reactive compound 10

8B5P の概要

• エントリーDOI: 10.2210/pdb8b5p/pdb
• 分子名称: 14-3-3 protein sigma, Estrogen Related Receptor gamma phosphopeptide, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: 14-3-3, hub-protein, dual-reactive compound, peptide binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28155.50

構造登録者

Somsen, B.A.,Ottmann, C. (登録日: 2022-09-23, 公開日: 2023-03-29, 最終更新日: 2024-02-07)

主引用文献

Somsen, B.A.,Schellekens, R.J.C.,Verhoef, C.J.A.,Arkin, M.R.,Ottmann, C.,Cossar, P.J.,Brunsveld, L.
Reversible Dual-Covalent Molecular Locking of the 14-3-3/ERR gamma Protein-Protein Interaction as a Molecular Glue Drug Discovery Approach.
J.Am.Chem.Soc., 145:6741-6752, 2023

PubMed Abstract: Molecules that stabilize protein-protein interactions (PPIs) are invaluable as tool compounds for biophysics and (structural) biology, and as starting points for molecular glue drug discovery. However, identifying initial starting points for PPI stabilizing matter is highly challenging, and chemical optimization is labor-intensive. Inspired by chemical crosslinking and reversible covalent fragment-based drug discovery, we developed an approach that we term "molecular locks" to rapidly access molecular glue-like tool compounds. These dual-covalent small molecules reversibly react with a nucleophilic amino acid on each of the partner proteins to dynamically crosslink the protein complex. The PPI between the hub protein 14-3-3 and estrogen-related receptor γ (ERRγ) was used as a pharmacologically relevant case study. Based on a focused library of dual-reactive small molecules, a molecular glue tool compound was rapidly developed. Biochemical assays and X-ray crystallographic studies validated the ternary covalent complex formation and overall PPI stabilization via dynamic covalent crosslinking. The molecular lock approach is highly selective for the specific 14-3-3/ERRγ complex, over other 14-3-3 complexes. This selectivity is driven by the interplay of molecular reactivity and molecular recognition of the composite PPI binding interface. The long lifetime of the dual-covalent locks enabled the selective stabilization of the 14-3-3/ERRγ complex even in the presence of several other competing 14-3-3 clients with higher intrinsic binding affinities. The molecular lock approach enables systematic, selective, and potent stabilization of protein complexes to support molecular glue drug discovery.

PubMed: 36926879
DOI: 10.1021/jacs.2c12781

実験手法

X-RAY DIFFRACTION (1.4 Å)