8B4I

Cryo-EM structure of the Neurospora crassa TOM core complex at 3.3 angstrom

8B4I の概要

• エントリーDOI: 10.2210/pdb8b4i/pdb
• EMDBエントリー: 15849
• 分子名称: Mitochondrial import receptor subunit Tom40, Mitochondrial import receptor subunit Tom22, Translocase of outer mitochondrial membrane subunit 5, ... (7 entities in total)
• 機能のキーワード: complex, outer membrane, mitochondria, membrane protein, tom, translocase, neurospora crassa
• 由来する生物種: Neurospora crassa; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 152330.21

構造登録者

Ornelas, P.,Kuehlbrandt, W. (登録日: 2022-09-20, 公開日: 2023-08-09, 最終更新日: 2023-08-30)

主引用文献

Ornelas, P.,Bausewein, T.,Martin, J.,Morgner, N.,Nussberger, S.,Kuhlbrandt, W.
Two conformations of the Tom20 preprotein receptor in the TOM holo complex.
Proc.Natl.Acad.Sci.USA, 120:e2301447120-e2301447120, 2023

PubMed Abstract: The TOM complex is the main entry point for precursor proteins (preproteins) into mitochondria. Preproteins containing targeting sequences are recognized by the TOM complex and imported into mitochondria. We have determined the structure of the TOM core complex from by single-particle electron cryomicroscopy at 3.3 Å resolution, showing its interaction with a bound preprotein at 4 Å resolution, and of the TOM holo complex including the Tom20 receptor at 6 to 7 Å resolution. TOM is a transmembrane complex consisting of two β-barrels, three receptor subunits, and three short transmembrane subunits. Tom20 has a transmembrane helix and a receptor domain on the cytoplasmic side. We propose that Tom20 acts as a dynamic gatekeeper, guiding preproteins into the pores of the TOM complex. We analyze the interactions of Tom20 with other TOM subunits, present insights into the structure of the TOM holo complex, and suggest a translocation mechanism.

PubMed: 37579144
DOI: 10.1073/pnas.2301447120

実験手法

ELECTRON MICROSCOPY (3.32 Å)