8B48

Structure of Lentithecium fluviatile carbohydrate esterase from the CE15 family (LfCE15C)

8B48 の概要

• エントリーDOI: 10.2210/pdb8b48/pdb
• 分子名称: Carbohydrate esterase family 15 protein, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: lignocellulose degradation, hydrolase
• 由来する生物種: Lentithecium fluviatile
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 176637.55

構造登録者

Scholzen, K.,Mazurkewich, S.,Poulsen, J.C.N.,Larsbrink, J.,Lo Leggio, L. (登録日: 2022-09-20, 公開日: 2023-06-07, 最終更新日: 2024-11-20)

主引用文献

Mazurkewich, S.,Scholzen, K.C.,Brusch, R.H.,Poulsen, J.C.N.,Theibich, Y.,Huttner, S.,Olsson, L.,Larsbrink, J.,Lo Leggio, L.
Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans.
Acta Crystallogr D Struct Biol, 79:545-555, 2023

PubMed Abstract: In plant cell walls, covalent bonds between polysaccharides and lignin increase recalcitrance to degradation. Ester bonds are known to exist between glucuronic acid moieties on glucuronoxylan and lignin, and these can be cleaved by glucuronoyl esterases (GEs) from carbohydrate esterase family 15 (CE15). GEs are found in both bacteria and fungi, and some microorganisms also encode multiple GEs, although the reason for this is still not fully clear. The fungus Lentithecium fluviatile encodes three CE15 enzymes, of which two have previously been heterologously produced, although neither was active on the tested model substrate. Here, one of these, LfCE15C, has been investigated in detail using a range of model and natural substrates and its structure has been solved using X-ray crystallography. No activity could be verified on any tested substrate, but biophysical assays indicate an ability to bind to complex carbohydrate ligands. The structure further suggests that this enzyme, which possesses an intact catalytic triad, might be able to bind and act on more extensively decorated xylan chains than has been reported for other CE15 members. It is speculated that rare glucuronoxylans decorated at the glucuronic acid moiety may be the true targets of LfCE15C and other CE15 family members with similar sequence characteristics.

PubMed: 37227091
DOI: 10.1107/S205979832300325X

実験手法

X-RAY DIFFRACTION (2.65 Å)