8B43

Crystal structure of ferrioxamine transporter

8B43 の概要

• エントリーDOI: 10.2210/pdb8b43/pdb
• 分子名称: Metal-pseudopaline receptor CntO, SULFATE ION, octyl beta-D-glucopyranoside, ... (5 entities in total)
• 機能のキーワード: siderophore tonb-dependent transporter pseudomonas aeruginosa iron, membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 92632.74

構造登録者

Josts, I.,Tidow, H. (登録日: 2022-09-19, 公開日: 2023-04-26, 最終更新日: 2026-01-21)

主引用文献

Chan, D.C.K.,Josts, I.,Koteva, K.,Wright, G.D.,Tidow, H.,Burrows, L.L.
Interactions of TonB-dependent transporter FoxA with siderophores and antibiotics that affect binding, uptake, and signal transduction.
Proc.Natl.Acad.Sci.USA, 120:e2221253120-e2221253120, 2023

PubMed Abstract: The outer membrane of gram-negative bacteria prevents many antibiotics from reaching intracellular targets. However, some antimicrobials can take advantage of iron import transporters to cross this barrier. We showed previously that the thiopeptide antibiotic thiocillin exploits the nocardamine xenosiderophore transporter, FoxA, of the opportunistic pathogen for uptake. Here, we show that FoxA also transports the xenosiderophore bisucaberin and describe at 2.5 Å resolution the crystal structure of bisucaberin bound to FoxA. Bisucaberin is distinct from other siderophores because it forms a 3:2 rather than 1:1 siderophore-iron complex. Mutations in a single extracellular loop of FoxA differentially affected nocardamine, thiocillin, and bisucaberin binding, uptake, and signal transduction. These results show that in addition to modulating ligand binding, the extracellular loops of siderophore transporters are of fundamental importance for controlling ligand uptake and its regulatory consequences, which have implications for the development of siderophore-antibiotic conjugates to treat difficult infections.

PubMed: 37043535
DOI: 10.1073/pnas.2221253120

実験手法

X-RAY DIFFRACTION (2.49 Å)