8B3L

Hen Egg White Lysozyme 2s in situ crystallization

8B3L の概要

• エントリーDOI: 10.2210/pdb8b3l/pdb
• 分子名称: Lysozyme, CHLORIDE ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14732.37

構造登録者

Henkel, A.,Galchenkova, M.,Yefanov, O.,Hakanpaeae, J.,Chapman, H.N.,Oberthuer, D. (登録日: 2022-09-16, 公開日: 2022-12-21, 最終更新日: 2024-11-13)

主引用文献

Henkel, A.,Galchenkova, M.,Maracke, J.,Yefanov, O.,Klopprogge, B.,Hakanpaa, J.,Mesters, J.R.,Chapman, H.N.,Oberthuer, D.
JINXED: just in time crystallization for easy structure determination of biological macromolecules.
Iucrj, 10:253-260, 2023

PubMed Abstract: Macromolecular crystallography is a well established method in the field of structural biology and has led to the majority of known protein structures to date. After focusing on static structures, the method is now under development towards the investigation of protein dynamics through time-resolved methods. These experiments often require multiple handling steps of the sensitive protein crystals, e.g. for ligand-soaking and cryo-protection. These handling steps can cause significant crystal damage, and hence reduce data quality. Furthermore, in time-resolved experiments based on serial crystallography, which use micrometre-sized crystals for short diffusion times of ligands, certain crystal morphologies with small solvent channels can prevent sufficient ligand diffusion. Described here is a method that combines protein crystallization and data collection in a novel one-step process. Corresponding experiments were successfully performed as a proof-of-principle using hen egg-white lysozyme and crystallization times of only a few seconds. This method, called JINXED (Just IN time Crystallization for Easy structure Determination), promises high-quality data due to the avoidance of crystal handling and has the potential to enable time-resolved experiments with crystals containing small solvent channels by adding potential ligands to the crystallization buffer, simulating traditional co-crystallization approaches.

PubMed: 36892542
DOI: 10.1107/S2052252523001653

実験手法

X-RAY DIFFRACTION (1.71 Å)