8B3B

Structure of metacyclic VSG (mVSG) 531 from Trypanosoma brucei

8B3B の概要

• エントリーDOI: 10.2210/pdb8b3b/pdb
• 分子名称: Variant surface glycoprotein 531, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: vsg, trypanosome, trypanosoma, metacyclic, membrane protein
• 由来する生物種: Trypanosoma brucei; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 319414.04

構造登録者

Chandra, M.,Stebbins, C.E. (登録日: 2022-09-16, 公開日: 2023-01-25, 最終更新日: 2024-10-23)

主引用文献

Chandra, M.,Dakovic, S.,Foti, K.,Zeelen, J.P.,van Straaten, M.,Aresta-Branco, F.,Tihon, E.,Lubbehusen, N.,Ruppert, T.,Glover, L.,Papavasiliou, F.N.,Stebbins, C.E.
Structural similarities between the metacyclic and bloodstream form variant surface glycoproteins of the African trypanosome.
Plos Negl Trop Dis, 17:e0011093-e0011093, 2023

PubMed Abstract: During infection of mammalian hosts, African trypanosomes thwart immunity using antigenic variation of the dense Variant Surface Glycoprotein (VSG) coat, accessing a large repertoire of several thousand genes and pseudogenes, and switching to antigenically distinct copies. The parasite is transferred to mammalian hosts by the tsetse fly. In the salivary glands of the fly, the pathogen adopts the metacyclic form and expresses a limited repertoire of VSG genes specific to that developmental stage. It has remained unknown whether the metacyclic VSGs possess distinct properties associated with this particular and discrete phase of the parasite life cycle. We present here three novel metacyclic form VSG N-terminal domain crystal structures (mVSG397, mVSG531, and mVSG1954) and show that they mirror closely in architecture, oligomerization, and surface diversity the known classes of bloodstream form VSGs. These data suggest that the mVSGs are unlikely to be a specialized subclass of VSG proteins, and thus could be poor candidates as the major components of prophylactic vaccines against trypanosomiasis.

PubMed: 36780870
DOI: 10.1371/journal.pntd.0011093

実験手法

X-RAY DIFFRACTION (1.949 Å)