8B3A

catalytic amyloid fibril formed by Ac-LHLHLRL-amide

Summary for 8B3A

• Entry DOI: 10.2210/pdb8b3a/pdb
• EMDB information: 15824
• Descriptor: ACE-LEU-HIS-LEU-HIS-LEU-ARG-LEU-NH2 (1 entity in total)
• Functional Keywords: catalytic amyloid fibril, protein fibril, zinc binding, prion
• Biological source: Homo sapiens
• Total number of polymer chains: 30
• Total formula weight: 27845.37

Authors

Heerde, T.,Schmidt, M.,Faendrich, M. (deposition date: 2022-09-16, release date: 2023-04-05, Last modification date: 2024-11-20)

Primary citation

Heerde, T.,Bansal, A.,Schmidt, M.,Fandrich, M.
Cryo-EM structure of a catalytic amyloid fibril.
Sci Rep, 13:4070-4070, 2023

PubMed Abstract: Catalytic amyloid fibrils are novel types of bioinspired, functional materials that combine the chemical and mechanical robustness of amyloids with the ability to catalyze a certain chemical reaction. In this study we used cryo-electron microcopy to analyze the amyloid fibril structure and the catalytic center of amyloid fibrils that hydrolyze ester bonds. Our findings show that catalytic amyloid fibrils are polymorphic and consist of similarly structured, zipper-like building blocks that consist of mated cross-β sheets. These building blocks define the fibril core, which is decorated by a peripheral leaflet of peptide molecules. The observed structural arrangement differs from previously described catalytic amyloid fibrils and yielded a new model of the catalytic center.

PubMed: 36906667
DOI: 10.1038/s41598-023-30711-y

Experimental method

ELECTRON MICROSCOPY (3.8 Å)