8B2V

Millisecond cryo-trapping by the spitrobot crystal plunger, CTX-M-14 E166A Ampicillin, 1 sec

Summary for 8B2V

• Entry DOI: 10.2210/pdb8b2v/pdb
• Descriptor: Beta-lactamase, (2R,4S)-2-[(1R)-1-{[(2R)-2-amino-2-phenylacetyl]amino}-2-oxoethyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid (3 entities in total)
• Functional Keywords: beta-lactamase, ctx-m-14, time-resolved crystallography, hydrolase
• Biological source: Klebsiella pneumoniae
• Total number of polymer chains: 1
• Total formula weight: 27951.54

Authors

Mehrabi, P.,Sung, S.,von Stetten, D.,Prester, A.,Hatton, C.E.,Kleine-Doepke, S.,Berkes, A.,Gore, G.,Leimkohl, J.P.,Schikora, H.,Kollewe, M.,Rohde, H.,Wilmanns, M.,Tellkamp, F.,Schulz, E.C. (deposition date: 2022-09-14, release date: 2023-05-24, Last modification date: 2024-10-16)

Primary citation

Mehrabi, P.,Sung, S.,von Stetten, D.,Prester, A.,Hatton, C.E.,Kleine-Dopke, S.,Berkes, A.,Gore, G.,Leimkohl, J.P.,Schikora, H.,Kollewe, M.,Rohde, H.,Wilmanns, M.,Tellkamp, F.,Schulz, E.C.
Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography.
Nat Commun, 14:2365-2365, 2023

PubMed Abstract: We introduce the spitrobot, a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, where the crystals are kept in a humidity and temperature-controlled environment, then reactions are initiated via the liquid application method (LAMA) and plunging into liquid nitrogen is initiated after an electronically set delay time to cryo-trap intermediate states. High-magnification images are automatically recorded before and after droplet deposition, prior to plunging. The SPINE-standard sample holder is directly plunged into a storage puck, enabling compatibility with high-throughput infrastructure. Here we demonstrate binding of glucose and 2,3-butanediol in microcrystals of xylose isomerase, and of avibactam and ampicillin in microcrystals of the extended spectrum beta-lactamase CTX-M-14. We also trap reaction intermediates and conformational changes in macroscopic crystals of tryptophan synthase to demonstrate that the spitrobot enables insight into catalytic events.

PubMed: 37185266
DOI: 10.1038/s41467-023-37834-w

Experimental method

X-RAY DIFFRACTION (1.73 Å)