8B12

cryo-EM structure of carboxysomal mini-shell: icosahedral assembly from CsoS4A/1A and CsoS2 co-expression (T = 9)

8B12 の概要

• エントリーDOI: 10.2210/pdb8b12/pdb
• EMDBエントリー: 15798 15799 15801
• 分子名称: Major carboxysome shell protein CsoS1A, Carboxysome shell vertex protein CsoS4A, Carboxysome assembly protein CsoS2B, ... (4 entities in total)
• 機能のキーワード: carboxysome, shell, icosahedral symmetry, structural protein
• 由来する生物種: Halothiobacillus neapolitanus; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 180190.14

構造登録者

Ni, T.,Jiang, Q.,Liu, L.N.,Zhang, P. (登録日: 2022-09-09, 公開日: 2023-08-23, 最終更新日: 2024-11-06)

主引用文献

Ni, T.,Jiang, Q.,Ng, P.C.,Shen, J.,Dou, H.,Zhu, Y.,Radecke, J.,Dykes, G.F.,Huang, F.,Liu, L.N.,Zhang, P.
Intrinsically disordered CsoS2 acts as a general molecular thread for alpha-carboxysome shell assembly.
Nat Commun, 14:5512-5512, 2023

PubMed Abstract: Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal "molecular thread" stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications.

PubMed: 37679318
DOI: 10.1038/s41467-023-41211-y

実験手法

ELECTRON MICROSCOPY (1.86 Å)