8B0Q

Deinococcus radiodurans UvrC C-terminal half

8B0Q の概要

• エントリーDOI: 10.2210/pdb8b0q/pdb
• 分子名称: UvrABC system protein C (2 entities in total)
• 機能のキーワード: dna repair; nucleotide excision repair; bacteria; uvrc, dna binding protein
• 由来する生物種: Deinococcus radiodurans R1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28065.06

構造登録者

Timmins, J.,Stelter, M. (登録日: 2022-09-08, 公開日: 2023-02-15, 最終更新日: 2024-02-07)

主引用文献

Seck, A.,De Bonis, S.,Stelter, M.,Okvist, M.,Senarisoy, M.,Hayek, M.R.,Le Roy, A.,Martin, L.,Saint-Pierre, C.,Silveira, C.M.,Gasparutto, D.,Todorovic, S.,Ravanat, J.L.,Timmins, J.
Structural and functional insights into the activation of the dual incision activity of UvrC, a key player in bacterial NER.
Nucleic Acids Res., 51:2931-2949, 2023

PubMed Abstract: Bacterial nucleotide excision repair (NER), mediated by the UvrA, UvrB and UvrC proteins is a multistep, ATP-dependent process, that is responsible for the removal of a very wide range of chemically and structurally diverse DNA lesions. DNA damage removal is performed by UvrC, an enzyme possessing a dual endonuclease activity, capable of incising the DNA on either side of the damaged site to release a short single-stranded DNA fragment containing the lesion. Using biochemical and biophysical approaches, we have probed the oligomeric state, UvrB- and DNA-binding abilities and incision activities of wild-type and mutant constructs of UvrC from the radiation resistant bacterium, Deinococcus radiodurans. Moreover, by combining the power of new structure prediction algorithms and experimental crystallographic data, we have assembled the first model of a complete UvrC, revealing several unexpected structural motifs and in particular, a central inactive RNase H domain acting as a platform for the surrounding domains. In this configuration, UvrC is maintained in a 'closed' inactive state that needs to undergo a major rearrangement to adopt an 'open' active state capable of performing the dual incision reaction. Taken together, this study provides important insight into the mechanism of recruitment and activation of UvrC during NER.

PubMed: 36869664
DOI: 10.1093/nar/gkad108

実験手法

X-RAY DIFFRACTION (1.8 Å)