8AVT

Racemic protein crystal structure of aureocin A53 from Staphylococcus aureus in the presence of glycerol 3-phosphate

8AVT の概要

• エントリーDOI: 10.2210/pdb8avt/pdb
• 分子名称: D-Aureocin A53, Bacteriocin aureocin A53, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: bacteriocin, d-protein, racemic, mirror-image, antimicrobial protein
• 由来する生物種: Staphylococcus aureus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 24809.47

構造登録者

Lander, A.J.,Baumann, P.,Rizkallah, P.,Jin, Y.,Luk, L.Y.P. (登録日: 2022-08-26, 公開日: 2023-07-26, 最終更新日: 2026-03-04)

主引用文献

Lander, A.J.,Mercado, L.D.,Li, X.,Taily, I.M.,Findlay, B.L.,Jin, Y.,Luk, L.Y.P.
Roles of inter- and intramolecular tryptophan interactions in membrane-active proteins revealed by racemic protein crystallography.
Commun Chem, 6:154-154, 2023

PubMed Abstract: Tryptophan is frequently found on the surface of membrane-associated proteins that interact with the lipid membrane. However, because of their multifaceted interactions, it is difficult to pinpoint the structure-activity relationship of each tryptophan residue. Here, we describe the use of racemic protein crystallography to probe dedicated tryptophan interactions of a model tryptophan-rich bacteriocin aureocin A53 (AucA) by inclusion and/or exclusion of potential ligands. In the presence of tetrahedral anions that are isosteric to the head group of phospholipids, distinct tryptophan H-bond networks were revealed. H-bond donation by W40 was critical for antibacterial activity, as its substitution by 1-methyltryptophan resulted in substantial loss of activity against bacterial clinical isolates. Meanwhile, exclusion of tetrahedral ions revealed that W3 partakes in formation of a dimeric interface, thus suggesting that AucA is dimeric in solution and dissociated to interact with the phosphate head group in the presence of the lipid membrane. Based on these findings, we could predict the tryptophan residue responsible for activity as well as the oligomeric state of a distant homologue lacticin Q (48%).

PubMed: 37464011
DOI: 10.1038/s42004-023-00953-y

実験手法

X-RAY DIFFRACTION (1.2 Å)