8ANV

Crystal structure of phi3T_93 and phi3T AimX complex

8ANV の概要

• エントリーDOI: 10.2210/pdb8anv/pdb
• 分子名称: YopN. Phi3T_93, Arbitrium putative lysogeny regulator, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: arbitrium, phi3t, lysis-lysogeny decission, viral protein
• 由来する生物種: Bacillus phage phi3T; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 31410.26

構造登録者

Zamora-Caballero, S.,Marina, A. (登録日: 2022-08-05, 公開日: 2023-10-25, 最終更新日: 2024-01-17)

主引用文献

Zamora-Caballero, S.,Chmielowska, C.,Quiles-Puchalt, N.,Brady, A.,Del Sol, F.G.,Mancheno-Bonillo, J.,Felipe-Ruiz, A.,Meijer, W.J.J.,Penades, J.R.,Marina, A.
Antagonistic interactions between phage and host factors control arbitrium lysis-lysogeny decision.
Nat Microbiol, 9:161-172, 2024

PubMed Abstract: Phages can use a small-molecule communication arbitrium system to coordinate lysis-lysogeny decisions, but the underlying mechanism remains unknown. Here we determined that the arbitrium system in Bacillus subtilis phage phi3T modulates the bacterial toxin-antitoxin system MazE-MazF to regulate the phage life cycle. We show that phi3T expresses AimX and YosL, which bind to and inactivate MazF. AimX also inhibits the function of phi3T_93, a protein that promotes lysogeny by binding to MazE and releasing MazF. Overall, these mutually exclusive interactions promote the lytic cycle of the phage. After several rounds of infection, the phage-encoded AimP peptide accumulates intracellularly and inactivates the phage antiterminator AimR, a process that eliminates aimX expression from the aimP promoter. Therefore, when AimP increases, MazF activity promotes reversion back to lysogeny, since AimX is absent. Altogether, our study reveals the evolutionary strategy used by arbitrium to control lysis-lysogeny by domesticating and fine-tuning a phage-defence mechanism.

PubMed: 38177302
DOI: 10.1038/s41564-023-01550-4

実験手法

X-RAY DIFFRACTION (2.2 Å)