8AN2

S-layer protein SlaA from Sulfolobus acidocaldarius at pH 10.0

これはPDB形式変換不可エントリーです。

8AN2 の概要

• エントリーDOI: 10.2210/pdb8an2/pdb
• 関連するPDBエントリー: 7ZCX
• EMDBエントリー: 15530
• 分子名称: S-layer protein A, alpha-D-mannopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: s-layer, dimer, n-glycosylation, structural protein
• 由来する生物種: Sulfolobus acidocaldarius DSM 639
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 163777.09

構造登録者

Gambelli, L.,Isupov, M.N.,Daum, B. (登録日: 2022-08-04, 公開日: 2023-08-16, 最終更新日: 2024-11-13)

主引用文献

Gambelli, L.,McLaren, M.,Conners, R.,Sanders, K.,Gaines, M.C.,Clark, L.,Gold, V.A.M.,Kattnig, D.,Sikora, M.,Hanus, C.,Isupov, M.N.,Daum, B.
Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius.
Elife, 13:-, 2024

PubMed Abstract: Surface layers (S-layers) are resilient two-dimensional protein lattices that encapsulate many bacteria and most archaea. In archaea, S-layers usually form the only structural component of the cell wall and thus act as the final frontier between the cell and its environment. Therefore, S-layers are crucial for supporting microbial life. Notwithstanding their importance, little is known about archaeal S-layers at the atomic level. Here, we combined single-particle cryo electron microscopy, cryo electron tomography, and Alphafold2 predictions to generate an atomic model of the two-component S-layer of . The outer component of this S-layer (SlaA) is a flexible, highly glycosylated, and stable protein. Together with the inner and membrane-bound component (SlaB), they assemble into a porous and interwoven lattice. We hypothesise that jackknife-like conformational changes in SlaA play important roles in S-layer assembly.

PubMed: 38251732
DOI: 10.7554/eLife.84617

実験手法

ELECTRON MICROSCOPY (3.2 Å)