8AMU

RepB pMV158 OBD domain bound to DDR region

8AMU の概要

• エントリーDOI: 10.2210/pdb8amu/pdb
• 分子名称: Replication protein RepB, DNA (5'-D(*TP*CP*GP*GP*CP*GP*AP*CP*TP*TP*TP*TP*CP*GP*GP*CP*GP*AP*CP*TP*TP*TP*T)-3'), DNA (5'-D(*AP*AP*AP*AP*GP*TP*CP*GP*CP*CP*GP*AP*AP*AP*AP*GP*TP*CP*GP*CP*CP*GP*A)-3'), ... (5 entities in total)
• 機能のキーワード: rolling circle replication, repb, pmv158, ddr region, replication
• 由来する生物種: Streptococcus agalactiae; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 94061.88

構造登録者

Amodio, J.,Machon, C.,Boer, R.D.,Ruiz-Maso, J.A.,del Solar, G.,Coll, M. (登録日: 2022-08-04, 公開日: 2023-02-08, 最終更新日: 2024-02-07)

主引用文献

Machon, C.,Ruiz-Maso, J.A.,Amodio, J.,Boer, D.R.,Bordanaba-Ruiseco, L.,Bury, K.,Konieczny, I.,Del Solar, G.,Coll, M.
Structures of pMV158 replication initiator RepB with and without DNA reveal a flexible dual-function protein.
Nucleic Acids Res., 51:1458-1472, 2023

PubMed Abstract: DNA replication is essential to all living organisms as it ensures the fidelity of genetic material for the next generation of dividing cells. One of the simplest replication initiation mechanisms is the rolling circle replication. In the streptococcal plasmid pMV158, which confers antibiotic resistance to tetracycline, replication initiation is catalysed by RepB protein. The RepB N-terminal domain or origin binding domain binds to the recognition sequence (bind locus) of the double-strand origin of replication and cleaves one DNA strand at a specific site within the nic locus. Using biochemical and crystallographic analyses, here we show how the origin binding domain recognises and binds to the bind locus using structural elements removed from the active site, namely the recognition α helix, and a β-strand that organises upon binding. A new hexameric structure of full-length RepB that highlights the great flexibility of this protein is presented, which could account for its ability to perform different tasks, namely bind to two distinct loci and cleave one strand of DNA at the plasmid origin.

PubMed: 36688326
DOI: 10.1093/nar/gkac1271

実験手法

X-RAY DIFFRACTION (3 Å)