8AMN

Crystal structure of AUGUGGCAU duplex with strontium ions

8AMN の概要

• エントリーDOI: 10.2210/pdb8amn/pdb
• 関連するPDBエントリー: 8AMG 8AMI 8AMJ 8AMK 8AML 8AMM
• 分子名称: RNA (5'-R(*AP*UP*GP*UP*GP*GP*CP*AP*U)-3'), STRONTIUM ION (3 entities in total)
• 機能のキーワード: ug wobble, ba ions, rna, ugg repeats
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 82277.05

構造登録者

Kiliszek, A.,Rypniewski, W. (登録日: 2022-08-03, 公開日: 2022-11-23, 最終更新日: 2024-02-07)

主引用文献

Kiliszek, A.,Pluta, M.,Bejger, M.,Rypniewski, W.
Structure and thermodynamics of a UGG motif interacting with Ba2+ and other metal ions: accommodating changes in the RNA structure and the presence of a G(syn)-G(syn) pair.
Rna, 29:44-54, 2022

PubMed Abstract: The self-complementary triplet 5'UGG3'/5'UGG3' is a particular structural motif containing a noncanonical G-G pair and two U·G wobble pairs. It constitutes a specific structural and electrostatic environment attracting metal ions, particularly Ba ions. Crystallographic research has shown that two Ba cations are located in the major groove of the helix and interact directly with the UGG triplet. A comparison with the unliganded structure has revealed global changes in the RNA structure in the presence of metal ions, whereas thermodynamic measurements have shown increased stability. Moreover, in the structure with Ba, an unusual noncanonical G()-G() pair is observed instead of the common G()-G(). We further elucidated the metal binding properties of the UGG/UGG triplet by performing crystallographic and thermodynamic studies using DSC and UV melting with other metal ions. The results explain the preferences of the UGG sequence for Ba cations and point to possible applications of this metal-binding propensity.

PubMed: 36319090
DOI: 10.1261/rna.079414.122

実験手法

X-RAY DIFFRACTION (2.46 Å)