8AJX
E. coli NfsA with Fumarate
Summary for 8AJX
| Entry DOI | 10.2210/pdb8ajx/pdb |
| Descriptor | Oxygen-insensitive NADPH nitroreductase, FLAVIN MONONUCLEOTIDE, FUMARIC ACID, ... (5 entities in total) |
| Functional Keywords | nitroreductase, inhibitor complex, oxidoreductase |
| Biological source | Escherichia coli |
| Total number of polymer chains | 1 |
| Total formula weight | 27467.15 |
| Authors | Day, M.A.,Jarrom, D.,White, S.A.,Hyde, E.I. (deposition date: 2022-07-28, release date: 2023-01-18, Last modification date: 2024-02-07) |
| Primary citation | Day, M.A.,Jarrom, D.,Rajah, N.,Searle, P.F.,Hyde, E.I.,White, S.A. Oxygen-insensitive nitroreductase E. coli NfsA, but not NfsB, is inhibited by fumarate. Proteins, 91:585-592, 2023 Cited by PubMed Abstract: Escherichia coli NfsA and NfsB are founding members of two flavoprotein families that catalyze the oxygen-insensitive reduction of nitroaromatics and quinones by NAD(P)H. This reduction is required for the activity of nitrofuran antibiotics and the enzymes have also been proposed for use with nitroaromatic prodrugs in cancer gene therapy and biocatalysis, but the roles of the proteins in vivo in bacteria are not known. NfsA is NADPH-specific whereas NfsB can also use NADH. The crystal structures of E. coli NfsA and NfsB and several analogs have been determined previously. In our crystal trials, we unexpectedly observed NfsA bound to fumarate. We here present the X-ray structure of the E. coli NfsA-fumarate complex and show that fumarate acts as a weak inhibitor of NfsA but not of NfsB. The structural basis of this differential inhibition is conserved in the two protein families and occurs at fumarate concentrations found in vivo, so impacting the efficacy of these proteins. PubMed: 36443029DOI: 10.1002/prot.26451 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.25 Å) |
Structure validation
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