8AHL

Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and large box)

8AHL の概要

• エントリーDOI: 10.2210/pdb8ahl/pdb
• EMDBエントリー: 15446
• 分子名称: Crescentin, Crescentin-specific megabody MB13 (2 entities in total)
• 機能のキーワード: cytoskeleton, cell shape, intermediate filaments, coiled coil, assembly, structural protein
• 由来する生物種: Caulobacter vibrioides; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 808583.25

構造登録者

Liu, Y.,Lowe, J. (登録日: 2022-07-22, 公開日: 2023-08-16, 最終更新日: 2024-10-09)

主引用文献

Liu, Y.,van den Ent, F.,Lowe, J.
Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin.
Proc.Natl.Acad.Sci.USA, 121:e2309984121-e2309984121, 2024

PubMed Abstract: The protein crescentin is required for the crescent shape of the freshwater bacterium (). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features with eukaryotic intermediate filament (IF) proteins, including the formation of static filaments based on long and parallel coiled coils, the protein's length, structural roles in cell and organelle shape determination and the presence of a coiled coil discontinuity called the "stutter." Here, we have used electron cryomicroscopy (cryo-EM) to determine the structure of the full-length protein and its filament, exploiting a crescentin-specific nanobody. The filament is formed by two strands, related by twofold symmetry, that each consist of two dimers, resulting in an octameric assembly. Crescentin subunits form longitudinal contacts head-to-head and tail-to-tail, making the entire filament non-polar. Using in vivo site-directed cysteine cross-linking, we demonstrated that contacts observed in the in vitro filament structure exist in cells. Electron cryotomography (cryo-ET) of cells expressing crescentin showed filaments on the concave side of the curved cells, close to the inner membrane, where they form a band. When comparing with current models of IF proteins and their filaments, which are also built from parallel coiled coil dimers and lack overall polarity, it emerges that IF proteins form head-to-tail longitudinal contacts in contrast to crescentin and hence several inter-dimer contacts in IFs have no equivalents in crescentin filaments. Our work supports the idea that intermediate filament-like proteins achieve their shared polymerization and mechanical properties through a variety of filament architectures.

PubMed: 38324567
DOI: 10.1073/pnas.2309984121

実験手法

ELECTRON MICROSCOPY (4.1 Å)