8AGQ

Crystal structure of anthocyanin-related GSTF8 from Populus trichocarpa in complex with (-)-catechin and glutathione

8AGQ の概要

• エントリーDOI: 10.2210/pdb8agq/pdb
• 分子名称: Glutathione transferase, GLUTATHIONE, (2~{S},3~{R})-2-[3,4-bis(oxidanyl)phenyl]-3,4-dihydro-2~{H}-chromene-3,5,7-triol, ... (5 entities in total)
• 機能のキーワード: anthocyanin, dehydratase, transferase
• 由来する生物種: Populus trichocarpa (black cottonwood)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25170.69

構造登録者

Eichenberger, M.,Hueppi, S.,Schwander, T.,Mittl, P.,Buller, M.R. (登録日: 2022-07-20, 公開日: 2023-08-30, 最終更新日: 2023-11-08)

主引用文献

Eichenberger, M.,Schwander, T.,Huppi, S.,Kreuzer, J.,Mittl, P.R.E.,Peccati, F.,Jimenez-Oses, G.,Naesby, M.,Buller, R.M.
The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis.
Nat Catal, 6:927-938, 2023

PubMed Abstract: Anthocyanins are ubiquitous plant pigments used in a variety of technological applications. Yet, after over a century of research, the penultimate biosynthetic step to anthocyanidins attributed to the action of leucoanthocyanidin dioxygenase has never been efficiently reconstituted outside plants, preventing the construction of heterologous cell factories. Through biochemical and structural analysis, here we show that anthocyanin-related glutathione transferases, currently implicated only in anthocyanin transport, catalyse an essential dehydration of the leucoanthocyanidin dioxygenase product, flavan-3,3,4-triol, to generate cyanidin. Building on this knowledge, introduction of anthocyanin-related glutathione transferases into a heterologous biosynthetic pathway in baker's yeast results in >35-fold increased anthocyanin production. In addition to unravelling the long-elusive anthocyanin biosynthesis, our findings pave the way for the colourants' heterologous microbial production and could impact the breeding of industrial and ornamental plants.

PubMed: 37881531
DOI: 10.1038/s41929-023-01018-y

実験手法

X-RAY DIFFRACTION (1.093 Å)