8AG4

Vaccinia C16 protein bound to Ku70/Ku80

8AG4 の概要

• エントリーDOI: 10.2210/pdb8ag4/pdb
• EMDBエントリー: 15414 15415 15416
• 分子名称: X-ray repair cross-complementing protein 6, X-ray repair cross-complementing protein 5, Protein C10 (3 entities in total)
• 機能のキーワード: c16 vaccinia virus protein ku70/ku80 dna binding inhibition, viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 244734.55

構造登録者

Rivera-Calzada, A.,Arribas-Bosacoma, R.,Pearl, L.H.,Llorca, O. (登録日: 2022-07-19, 公開日: 2022-11-09, 最終更新日: 2024-07-24)

主引用文献

Rivera-Calzada, A.,Arribas-Bosacoma, R.,Ruiz-Ramos, A.,Escudero-Bravo, P.,Boskovic, J.,Fernandez-Leiro, R.,Oliver, A.W.,Pearl, L.H.,Llorca, O.
Structural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus.
Nat Commun, 13:7062-7062, 2022

PubMed Abstract: Detection of cytosolic DNA is a central element of the innate immunity system against viral infection. The Ku heterodimer, a component of the NHEJ pathway of DNA repair in the nucleus, functions as DNA sensor that detects dsDNA of viruses that replicate in the cytoplasm. Vaccinia virus expresses two proteins, C4 and C16, that inactivate DNA sensing and enhance virulence. The structural basis for this is unknown. Here we determine the structure of the C16 - Ku complex using cryoEM. Ku binds dsDNA by a preformed ring but C16 sterically blocks this access route, abrogating binding to a dsDNA end and its insertion into DNA-PK, thereby averting signalling into the downstream innate immunity system. C4 replicates these activities using a domain with 54% identity to C16. Our results reveal how vaccinia virus subverts the capacity of Ku to recognize viral DNA.

PubMed: 36400800
DOI: 10.1038/s41467-022-34843-z

実験手法

ELECTRON MICROSCOPY (2.46 Å)