8AF7

Room temperature SSX crystal structure of CTX-M-14 (10K dataset)

Summary for 8AF7

• Entry DOI: 10.2210/pdb8af7/pdb
• Descriptor: Beta-lactamase, SULFATE ION (3 entities in total)
• Functional Keywords: beta-lactamase, serial crystallography, ssx, tapedrive, hydrolase
• Biological source: Klebsiella pneumoniae
• Total number of polymer chains: 1
• Total formula weight: 27867.38

Authors

Oberthuer, D.,Perbandt, M.,Prester, A.,Rohde, H.,Betzel, C.,Yefanov, O. (deposition date: 2022-07-15, release date: 2022-11-16, Last modification date: 2024-04-03)

Primary citation

Zielinski, K.A.,Prester, A.,Andaleeb, H.,Bui, S.,Yefanov, O.,Catapano, L.,Henkel, A.,Wiedorn, M.O.,Lorbeer, O.,Crosas, E.,Meyer, J.,Mariani, V.,Domaracky, M.,White, T.A.,Fleckenstein, H.,Sarrou, I.,Werner, N.,Betzel, C.,Rohde, H.,Aepfelbacher, M.,Chapman, H.N.,Perbandt, M.,Steiner, R.A.,Oberthuer, D.
Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Iucrj, 9:778-791, 2022

PubMed Abstract: Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physio-logical temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance ( CTX-M-14 β-lactamase, xylanase GH11 and urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

PubMed: 36381150
DOI: 10.1107/S2052252522010193

Experimental method

X-RAY DIFFRACTION (1.55 Å)