8AA2

Inactive levan utilisation machinery (utilisome) in the presence of levan fructo-oligosaccharides DP 15-25

8AA2 の概要

• エントリーDOI: 10.2210/pdb8aa2/pdb
• EMDBエントリー: 15288 15289 15290 15291
• 分子名称: Glycoside hydrolase family 32, DUF4960 domain-containing protein, SusD homolog, ... (9 entities in total)
• 機能のキーワード: membrane protein transporter, glycan transporter, suscd, utilisome, tonb dependent transporter, tbdt, transport protein, levan
• 由来する生物種: Bacteroides thetaiotaomicron VPI-5482; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 589573.71

構造登録者

White, J.B.R.,Silale, A.,Ranson, N.A.,van den Berg, B. (登録日: 2022-06-29, 公開日: 2023-06-07, 最終更新日: 2024-11-20)

主引用文献

White, J.B.R.,Silale, A.,Feasey, M.,Heunis, T.,Zhu, Y.,Zheng, H.,Gajbhiye, A.,Firbank, S.,Basle, A.,Trost, M.,Bolam, D.N.,van den Berg, B.,Ranson, N.A.
Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes.
Nature, 618:583-589, 2023

PubMed Abstract: Bacteroidetes are abundant members of the human microbiota, utilizing a myriad of diet- and host-derived glycans in the distal gut. Glycan uptake across the bacterial outer membrane of these bacteria is mediated by SusCD protein complexes, comprising a membrane-embedded barrel and a lipoprotein lid, which is thought to open and close to facilitate substrate binding and transport. However, surface-exposed glycan-binding proteins and glycoside hydrolases also play critical roles in the capture, processing and transport of large glycan chains. The interactions between these components in the outer membrane are poorly understood, despite being crucial for nutrient acquisition by our colonic microbiota. Here we show that for both the levan and dextran utilization systems of Bacteroides thetaiotaomicron, the additional outer membrane components assemble on the core SusCD transporter, forming stable glycan-utilizing machines that we term utilisomes. Single-particle cryogenic electron microscopy structures in the absence and presence of substrate reveal concerted conformational changes that demonstrate the mechanism of substrate capture, and rationalize the role of each component in the utilisome.

PubMed: 37286596
DOI: 10.1038/s41586-023-06146-w

実験手法

ELECTRON MICROSCOPY (3.1 Å)