8A8K

PAP phosphatase from Methanothermococcus thermolithotrophicus refined to 3.1 A

8A8K の概要

• エントリーDOI: 10.2210/pdb8a8k/pdb
• 分子名称: PAP phosphatase from Methanothermococcus thermolithotrophicus, ADENOSINE MONOPHOSPHATE, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: sulfate assimilation, methanogens, archaea, pap, thermophile, inorganic phosphate, amp, hydrolase, methane, detoxification, marine, manganese dependent, dhh-motif, recj
• 由来する生物種: Methanothermococcus thermolithotrophicus DSM 2095
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 230902.52

構造登録者

Jespersen, M.,Wagner, T. (登録日: 2022-06-23, 公開日: 2023-04-26, 最終更新日: 2024-05-01)

主引用文献

Jespersen, M.,Wagner, T.
Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus.
Nat Microbiol, 8:1227-1239, 2023

PubMed Abstract: Methanothermococcus thermolithotrophicus is the only known methanogen that grows on sulfate as its sole sulfur source, uniquely uniting methanogenesis and sulfate reduction. Here we use physiological, biochemical and structural analyses to provide a snapshot of the complete sulfate reduction pathway of this methanogenic archaeon. We find that later steps in this pathway are catalysed by atypical enzymes. PAPS (3'-phosphoadenosine 5'-phosphosulfate) released by APS kinase is converted into sulfite and 3'-phosphoadenosine 5'-phosphate (PAP) by a PAPS reductase that is similar to the APS reductases of dissimilatory sulfate reduction. A non-canonical PAP phosphatase then hydrolyses PAP. Finally, the F-dependent sulfite reductase converts sulfite to sulfide for cellular assimilation. While metagenomic and metatranscriptomic studies suggest that the sulfate reduction pathway is present in several methanogens, the sulfate assimilation pathway in M. thermolithotrophicus is distinct. We propose that this pathway was 'mix-and-matched' through the acquisition of assimilatory and dissimilatory enzymes from other microorganisms and then repurposed to fill a unique metabolic role.

PubMed: 37277534
DOI: 10.1038/s41564-023-01398-8

実験手法

X-RAY DIFFRACTION (3.1 Å)