8A5S

Crystal structure of light-activated DNA-binding protein EL222 from Erythrobacter litoralis crystallized in dark, measured illuminated.

これはPDB形式変換不可エントリーです。

8A5S の概要

• エントリーDOI: 10.2210/pdb8a5s/pdb
• 分子名称: Light-activated DNA-binding protein EL222, FLAVIN MONONUCLEOTIDE, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: light-activated transcription factor, dna binding protein, lov domain, transcription
• 由来する生物種: Erythrobacter litoralis HTCC2594
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24005.79

構造登録者

Koval, T.,Chaudhari, A.,Fuertes, G.,Andersson, I.,Dohnalek, J. (登録日: 2022-06-15, 公開日: 2023-07-05, 最終更新日: 2025-04-09)

主引用文献

Chaudhari, A.S.,Favier, A.,Tehrani, Z.A.,Koval, T.,Andersson, I.,Schneider, B.,Dohnalek, J.,Cerny, J.,Brutscher, B.,Fuertes, G.
Light-dependent flavin redox and adduct states control the conformation and DNA-binding activity of the transcription factor EL222.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: The activity of the light-oxygen-voltage/helix-turn-helix (LOV-HTH) photoreceptor EL222 is regulated through protein-protein and protein-DNA interactions, both triggered by photo-excitation of its flavin mononucleotide (FMN) cofactor. To gain molecular-level insight into the photocycle of EL222, we applied complementary methods: macromolecular X-ray crystallography (MX), nuclear magnetic resonance (NMR) spectroscopy, optical spectroscopies (infrared and UV-visible), molecular dynamics/metadynamics (MD/metaD) simulations, and protein engineering using noncanonical amino acids. Kinetic experiments provided evidence for two distinct EL222 conformations (lit1 and lit2) that become sequentially populated under illumination. These two lit states were assigned to covalently bound N5 protonated, and noncovalently bound hydroquinone forms of FMN, respectively. Only subtle structural differences were observed between the monomeric forms of all three EL222 species (dark, lit1, and lit2). While the dark state is largely monomeric, both lit states undergo monomer-dimer exchange. Furthermore, molecular modeling revealed differential dynamics and interdomain separation times arising from the three FMN states (oxidized, adduct, and reduced). Unexpectedly, all three EL222 species can associate with DNA, but only upon blue-light irradiation, a high population of stable complexes is obtained. Overall, we propose a model of EL222 activation where photoinduced changes in the FMN moiety shift the population equilibrium toward an open conformation that favors self-association and DNA-binding.

PubMed: 40119733
DOI: 10.1093/nar/gkaf215

実験手法

X-RAY DIFFRACTION (1.85 Å)