8A0H

Crystal structure of the E25A mutant of the Orange Carotenoid Protein X from Gloeobacter kilaueensis JS1 complexed with echinenone

Summary for 8A0H

• Entry DOI: 10.2210/pdb8a0h/pdb
• Descriptor: OCP N-terminal domain-containing protein, beta,beta-caroten-4-one, SULFATE ION, ... (4 entities in total)
• Functional Keywords: carotenoid-binding protein, carotenoid transport, cbp, gloeobacter kilaueensis, transport protein, nuclear transport factor 2
• Biological source: Gloeobacter kilaueensis JS1
• Total number of polymer chains: 1
• Total formula weight: 36560.77

Authors

Boyko, K.M.,Slonimskiy, Y.B.,Zupnik, A.O.,Varfolomeeva, L.A.,Maksimov, E.G.,Sluchanko, N.N. (deposition date: 2022-05-27, release date: 2023-02-01, Last modification date: 2024-05-01)

Primary citation

Slonimskiy, Y.B.,Zupnik, A.O.,Varfolomeeva, L.A.,Boyko, K.M.,Maksimov, E.G.,Sluchanko, N.N.
A primordial Orange Carotenoid Protein: Structure, photoswitching activity and evolutionary aspects.
Int.J.Biol.Macromol., 222:167-180, 2022

PubMed Abstract: Cyanobacteria are photosynthesizing prokaryotes responsible for the Great Oxygenation Event on Earth ~2.5 Ga years ago. They use a specific photoprotective mechanism based on the 35-kDa photoactive Orange Carotenoid Protein (OCP), a promising target for developing novel optogenetic tools and for biomass engineering. The two-domain OCP presumably stems from domain fusion, yet the primitive thylakoid-less cyanobacteria Gloeobacter encodes a complete OCP. Its photosynthesis regulation lacks the so-called Fluorescence Recovery Protein (FRP), which in Synechocystis inhibits OCP-mediated phycobilisome fluorescence quenching, and Gloeobacter OCP belongs to the recently defined, heterogeneous clade OCPX (GlOCPX), the least characterized compared to OCP2 and especially OCP1 clades. Here, we describe the first crystal structure of OCPX, which explains unique functional adaptations of Gloeobacter OCPX compared to OCP1 from Synechocystis. We show that monomeric GlOCPX exploits a remarkable intramolecular locking mechanism stabilizing its dark-adapted state and exhibits drastically accelerated, less temperature-dependent recovery after photoactivation. While GlOCPX quenches Synechocystis phycobilisomes similar to Synechocystis OCP1, it evades interaction with and regulation by FRP from other species and likely uses alternative mechanisms for fluorescence recovery. This analysis of a primordial OCPX sheds light on its evolution, rationalizing renaming and subdivision of the OCPX clade into subclades - OCP3a, OCP3b, OCP3c.

PubMed: 36165868
DOI: 10.1016/j.ijbiomac.2022.09.131

Experimental method

X-RAY DIFFRACTION (1.73 Å)