8A09

A hexameric barrel state of a de novo coiled-coil assembly: CC-Type2-(QgLaId)4

8A09 の概要

• エントリーDOI: 10.2210/pdb8a09/pdb
• 分子名称: A hexameric barrel state of a de novo coiled-coil assembly: CC-Type2-(QgLaId)4, GLYCEROL (3 entities in total)
• 機能のキーワード: de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7202.43

構造登録者

Martin, F.J.O.,Rhys, G.G.,Dawson, W.M.,Woolfson, D.N. (登録日: 2022-05-27, 公開日: 2022-06-15, 最終更新日: 2024-11-13)

主引用文献

Dawson, W.M.,Shelley, K.L.,Fletcher, J.M.,Scott, D.A.,Lombardi, L.,Rhys, G.G.,LaGambina, T.J.,Obst, U.,Burton, A.J.,Cross, J.A.,Davies, G.,Martin, F.J.O.,Wiseman, F.J.,Brady, R.L.,Tew, D.,Wood, C.W.,Woolfson, D.N.
Differential sensing with arrays of de novo designed peptide assemblies.
Nat Commun, 14:383-383, 2023

PubMed Abstract: Differential sensing attempts to mimic the mammalian senses of smell and taste to identify analytes and complex mixtures. In place of hundreds of complex, membrane-bound G-protein coupled receptors, differential sensors employ arrays of small molecules. Here we show that arrays of computationally designed de novo peptides provide alternative synthetic receptors for differential sensing. We use self-assembling α-helical barrels (αHBs) with central channels that can be altered predictably to vary their sizes, shapes and chemistries. The channels accommodate environment-sensitive dyes that fluoresce upon binding. Challenging arrays of dye-loaded barrels with analytes causes differential fluorophore displacement. The resulting fluorimetric fingerprints are used to train machine-learning models that relate the patterns to the analytes. We show that this system discriminates between a range of biomolecules, drink, and diagnostically relevant biological samples. As αHBs are robust and chemically diverse, the system has potential to sense many analytes in various settings.

PubMed: 36693847
DOI: 10.1038/s41467-023-36024-y

実験手法

X-RAY DIFFRACTION (1.35 Å)