8A06

Flavobacterium infecting lipid-containing phage FLiP penton protein

Summary for 8A06

• Entry DOI: 10.2210/pdb8a06/pdb
• EMDB information: 15051
• Descriptor: Penton protein P12 (1 entity in total)
• Functional Keywords: bacteriophage, flavobacterium, lipid-containing, phage, flip, penton protein, virus
• Biological source: Flavobacterium phage FLiP
• Total number of polymer chains: 1
• Total formula weight: 16851.06

Authors

Rissanen, I.,Huiskonen, J.T. (deposition date: 2022-05-26, release date: 2022-12-14, Last modification date: 2024-07-24)

Primary citation

Kejzar, N.,Laanto, E.,Rissanen, I.,Abrishami, V.,Selvaraj, M.,Moineau, S.,Ravantti, J.,Sundberg, L.R.,Huiskonen, J.T.
Cryo-EM structure of ssDNA bacteriophage Phi CjT23 provides insight into early virus evolution.
Nat Commun, 13:7478-7478, 2022

PubMed Abstract: The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution.

PubMed: 36463224
DOI: 10.1038/s41467-022-35123-6

Experimental method

ELECTRON MICROSCOPY (4 Å)