8A01

Bacteriophage phiCjT23 major capsid protein trimer type 1

8A01 の概要

• エントリーDOI: 10.2210/pdb8a01/pdb
• EMDBエントリー: 15044
• 分子名称: Major capsid protein P5 (1 entity in total)
• 機能のキーワード: bacteriophage, phicjt23, virus
• 由来する生物種: Flavobacterium phage
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 78542.35

構造登録者

Rissanen, I.,Kejzar, N.,Huiskonen, J.T. (登録日: 2022-05-26, 公開日: 2022-12-14, 最終更新日: 2024-07-24)

主引用文献

Kejzar, N.,Laanto, E.,Rissanen, I.,Abrishami, V.,Selvaraj, M.,Moineau, S.,Ravantti, J.,Sundberg, L.R.,Huiskonen, J.T.
Cryo-EM structure of ssDNA bacteriophage Phi CjT23 provides insight into early virus evolution.
Nat Commun, 13:7478-7478, 2022

PubMed Abstract: The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution.

PubMed: 36463224
DOI: 10.1038/s41467-022-35123-6

実験手法

ELECTRON MICROSCOPY (3.2 Å)