7ZZL

Crystal structure of CYP106A1

7ZZL の概要

• エントリーDOI: 10.2210/pdb7zzl/pdb
• 分子名称: Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (9 entities in total)
• 機能のキーワード: cytochrome p450, oxidoreductase
• 由来する生物種: Priestia megaterium DSM 319
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 198505.96

構造登録者

Carius, Y.,Kiss, F.,Hutter, M.,Bernhardt, R.,Lancaster, C.R.D. (登録日: 2022-05-25, 公開日: 2023-04-12, 最終更新日: 2024-02-07)

主引用文献

Carius, Y.,Hutter, M.,Kiss, F.,Bernhardt, R.,Lancaster, C.R.D.
Structural comparison of the cytochrome P450 enzymes CYP106A1 and CYP106A2 provides insight into their differences in steroid conversion.
Febs Lett., 596:3133-3144, 2022

PubMed Abstract: Understanding the structural basis of the selectivity of steroid hydroxylation requires detailed structural and functional investigations on various steroid hydroxylases with different selectivities, such as the bacterial cytochrome P450 enzymes. Here, the crystal structure of the cytochrome P450 CYP106A1 from Priestia megaterium was solved. CYP106A1 exhibits a rare additional structural motif of a cytochrome P450, a sixth β-sheet. The protein was found in different unusual conformations corresponding to both open and closed forms even when crystallized without any known substrate. The structural comparison of CYP106A1 with the previously investigated CYP106A2, including docking studies for both isoforms with the substrate cortisol, reveals a completely different orientation of the steroid molecule in the active sites. This distinction convincingly explains the experimentally observed differences in substrate conversion and product formation by the two enzymes.

PubMed: 36151590
DOI: 10.1002/1873-3468.14502

実験手法

X-RAY DIFFRACTION (1.7 Å)