7ZTD

Non-muscle F-actin decorated with non-muscle tropomyosin 3.2

7ZTD の概要

• エントリーDOI: 10.2210/pdb7ztd/pdb
• EMDBエントリー: 14958
• 分子名称: actin, cytoplasmic 1, Non-muscle tropomyosin 3.2, ADENOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: actin, tropomyosin, non-muscle, complex, cytosolic protein
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 383707.59

構造登録者

Selvaraj, M.,Kokate, S.,Kogan, K.,Kotila, T.,Kremneva, E.,Lappalainen, P.,Huiskonen, J.T. (登録日: 2022-05-09, 公開日: 2023-01-11, 最終更新日: 2024-07-24)

主引用文献

Selvaraj, M.,Kokate, S.B.,Reggiano, G.,Kogan, K.,Kotila, T.,Kremneva, E.,DiMaio, F.,Lappalainen, P.,Huiskonen, J.T.
Structural basis underlying specific biochemical activities of non-muscle tropomyosin isoforms.
Cell Rep, 42:111900-111900, 2023

PubMed Abstract: The actin cytoskeleton is critical for cell migration, morphogenesis, endocytosis, organelle dynamics, and cytokinesis. To support diverse cellular processes, actin filaments form a variety of structures with specific architectures and dynamic properties. Key proteins specifying actin filaments are tropomyosins. Non-muscle cells express several functionally non-redundant tropomyosin isoforms, which differentially control the interactions of other proteins, including myosins and ADF/cofilin, with actin filaments. However, the underlying molecular mechanisms have remained elusive. By determining the cryogenic electron microscopy structures of actin filaments decorated by two functionally distinct non-muscle tropomyosin isoforms, Tpm1.6 and Tpm3.2, we reveal that actin filament conformation remains unaffected upon binding. However, Tpm1.6 and Tpm3.2 follow different paths along the actin filament major groove, providing an explanation for their incapability to co-polymerize on actin filaments. We also elucidate the molecular basis underlying specific roles of Tpm1.6 and Tpm3.2 in myosin II activation and protecting actin filaments from ADF/cofilin-catalyzed severing.

PubMed: 36586407
DOI: 10.1016/j.celrep.2022.111900

実験手法

ELECTRON MICROSCOPY (4.6 Å)