7ZQP

Tail tip of siphophage T5 : open cone after interaction with bacterial receptor FhuA

7ZQP の概要

• エントリーDOI: 10.2210/pdb7zqp/pdb
• EMDBエントリー: 14873
• 分子名称: Probable baseplate hub protein, Probable tape measure protein (2 entities in total)
• 機能のキーワード: bacteriophage, siphophage, t5, baseplate, viral protein
• 由来する生物種: Escherichia phage T5; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 716758.03

構造登録者

Linares, R.,Arnaud, C.A.,Effantin, G.,Darnault, C.,Epalle, N.,Boeri Erba, E.,Schoehn, G.,Breyton, C. (登録日: 2022-05-02, 公開日: 2023-02-08, 最終更新日: 2023-07-05)

主引用文献

Linares, R.,Arnaud, C.A.,Effantin, G.,Darnault, C.,Epalle, N.H.,Boeri Erba, E.,Schoehn, G.,Breyton, C.
Structural basis of bacteriophage T5 infection trigger and E. coli cell wall perforation.
Sci Adv, 9:eade9674-eade9674, 2023

PubMed Abstract: Most bacteriophages present a tail allowing host recognition, cell wall perforation, and viral DNA channeling from the capsid to the infected bacterium cytoplasm. The majority of tailed phages bear a long flexible tail () at the tip of which receptor binding proteins (RBPs) specifically interact with their host, triggering infection. In siphophage T5, the unique RBP is located at the extremity of a central fiber. We present the structures of T5 tail tip, determined by cryo-electron microscopy before and after interaction with its receptor, FhuA, reconstituted into nanodisc. These structures bring out the important conformational changes undergone by T5 tail tip upon infection, which include bending of T5 central fiber on the side of the tail tip, tail anchoring to the membrane, tail tube opening, and formation of a transmembrane channel. The data allow to detail the first steps of an otherwise undescribed infection mechanism.

PubMed: 36961893
DOI: 10.1126/sciadv.ade9674

実験手法

ELECTRON MICROSCOPY (3.6 Å)