7ZN9

Crystal structure of the light-driven inward proton pump xenorhodopsin BcXeR in the M state at pH 7.0 in the presence of sodium at 100K

7ZN9 の概要

• エントリーDOI: 10.2210/pdb7zn9/pdb
• 分子名称: xenorhodopsin, EICOSANE, OLEIC ACID, ... (5 entities in total)
• 機能のキーワード: retinal, rhodopsin, xenorhodopsin, ion transport, isomerization, photocycle, membrane protein
• 由来する生物種: Bacillus coahuilensis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 94318.27

構造登録者

Kovalev, K.,Tsybrov, F.,Alekseev, A.,Bourenkov, G.,Gordeliy, V. (登録日: 2022-04-20, 公開日: 2023-05-10, 最終更新日: 2024-02-07)

主引用文献

Kovalev, K.,Tsybrov, F.,Alekseev, A.,Shevchenko, V.,Soloviov, D.,Siletsky, S.,Bourenkov, G.,Agthe, M.,Nikolova, M.,von Stetten, D.,Astashkin, R.,Bukhdruker, S.,Chizhov, I.,Royant, A.,Kuzmin, A.,Gushchin, I.,Rosselli, R.,Rodriguez-Valera, F.,Ilyinskiy, N.,Rogachev, A.,Borshchevskiy, V.,Schneider, T.R.,Bamberg, E.,Gordeliy, V.
Mechanisms of inward transmembrane proton translocation.
Nat.Struct.Mol.Biol., 30:970-979, 2023

PubMed Abstract: Proton transport is indispensable for cell life. It is believed that molecular mechanisms of proton movement through different types of proton-conducting molecules have general universal features. However, elucidation of such mechanisms is a challenge. It requires true-atomic-resolution structures of all key proton-conducting states. Here we present a comprehensive function-structure study of a light-driven bacterial inward proton pump, xenorhodopsin, from Bacillus coahuilensis in all major proton-conducting states. The structures reveal that proton translocation is based on proton wires regulated by internal gates. The wires serve as both selectivity filters and translocation pathways for protons. The cumulative results suggest a general concept of proton translocation. We demonstrate the use of serial time-resolved crystallography at a synchrotron source with sub-millisecond resolution for rhodopsin studies, opening the door for principally new applications. The results might also be of interest for optogenetics since xenorhodopsins are the only alternative tools to fire neurons.

PubMed: 37386213
DOI: 10.1038/s41594-023-01020-9

実験手法

X-RAY DIFFRACTION (2.3 Å)