7ZKE

Mot1:TBP:DNA - pre-hydrolysis state

7ZKE の概要

• エントリーDOI: 10.2210/pdb7zke/pdb
• EMDBエントリー: 14762
• 分子名称: DNA (36-MER), Putative tata-box binding protein, Helicase-like protein, ... (7 entities in total)
• 機能のキーワード: swi2/snf2 protein, remodeler, transcription initiation, transcription
• 由来する生物種: Chaetomium thermophilum; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 264190.32

構造登録者

Woike, S.,Eustermann, S.,Jung, J.,Wenzl, S.J.,Hagemann, G.,Bartho, J.D.,Lammens, K.,Butryn, A.,Herzog, F.,Hopfner, K.-P. (登録日: 2022-04-12, 公開日: 2023-04-26, 最終更新日: 2024-07-24)

主引用文献

Woike, S.,Eustermann, S.,Jung, J.,Wenzl, S.J.,Hagemann, G.,Bartho, J.,Lammens, K.,Butryn, A.,Herzog, F.,Hopfner, K.P.
Structural basis for TBP displacement from TATA box DNA by the Swi2/Snf2 ATPase Mot1.
Nat.Struct.Mol.Biol., 30:640-649, 2023

PubMed Abstract: The Swi2/Snf2 family transcription regulator Modifier of Transcription 1 (Mot1) uses adenosine triphosphate (ATP) to dissociate and reallocate the TATA box-binding protein (TBP) from and between promoters. To reveal how Mot1 removes TBP from TATA box DNA, we determined cryogenic electron microscopy structures that capture different states of the remodeling reaction. The resulting molecular video reveals how Mot1 dissociates TBP in a process that, intriguingly, does not require DNA groove tracking. Instead, the motor grips DNA in the presence of ATP and swings back after ATP hydrolysis, moving TBP to a thermodynamically less stable position on DNA. Dislodged TBP is trapped by a chaperone element that blocks TBP's DNA binding site. Our results show how Swi2/Snf2 proteins can remodel protein-DNA complexes through DNA bending without processive DNA tracking and reveal mechanistic similarities to RNA gripping DEAD box helicases and RIG-I-like immune sensors.

PubMed: 37106137
DOI: 10.1038/s41594-023-00966-0

実験手法

ELECTRON MICROSCOPY (3.6 Å)