7ZJ7

X-31 Hemagglutinin Precursor HA0 at pH 4.8

7ZJ7 の概要

• エントリーDOI: 10.2210/pdb7zj7/pdb
• 関連するPDBエントリー: 7ZJ6
• EMDBエントリー: 14743
• 分子名称: Hemagglutinin,Fibritin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: virus envelope, receptor binding, membrane fusion, viral protein
• 由来する生物種: Influenza A virus (A/Aichi/2/1968(H3N2)); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 193248.87

構造登録者

Garcia-Moro, E.,Rosenthal, P.B. (登録日: 2022-04-08, 公開日: 2022-08-17, 最終更新日: 2024-10-16)

主引用文献

Garcia-Moro, E.,Zhang, J.,Calder, L.J.,Brown, N.R.,Gamblin, S.J.,Skehel, J.J.,Rosenthal, P.B.
Reversible structural changes in the influenza hemagglutinin precursor at membrane fusion pH.
Proc.Natl.Acad.Sci.USA, 119:e2208011119-e2208011119, 2022

PubMed Abstract: The subunits of the influenza hemagglutinin (HA) trimer are synthesized as single-chain precursors (HA0s) that are proteolytically cleaved into the disulfide-linked polypeptides HA1 and HA2. Cleavage is required for activation of membrane fusion at low pH, which occurs at the beginning of infection following transfer of cell-surface-bound viruses into endosomes. Activation results in extensive changes in the conformation of cleaved HA. To establish the overall contribution of cleavage to the mechanism of HA-mediated membrane fusion, we used cryogenic electron microscopy (cryo-EM) to directly image HA0 at neutral and low pH. We found extensive pH-induced structural changes, some of which were similar to those described for intermediates in the refolding of cleaved HA at low pH. They involve a partial extension of the long central coiled coil formed by melting of the preexisting secondary structure, threading it between the membrane-distal domains, and subsequent refolding as extended helices. The fusion peptide, covalently linked at its N terminus, adopts an amphipathic helical conformation over part of its length and is repositioned and packed against a complementary surface groove of conserved residues. Furthermore, and in contrast to cleaved HA, the changes in HA0 structure at low pH are reversible on reincubation at neutral pH. We discuss the implications of covalently restricted HA0 refolding for the cleaved HA conformational changes that mediate membrane fusion and for the action of antiviral drug candidates and cross-reactive anti-HA antibodies that can block influenza infectivity.

PubMed: 35939703
DOI: 10.1073/pnas.2208011119

実験手法

ELECTRON MICROSCOPY (3.95 Å)