7ZI0

Structure of human Smoothened in complex with cholesterol and SAG

Summary for 7ZI0

Entry DOI	10.2210/pdb7zi0/pdb
Descriptor	Smoothened homolog,Soluble cytochrome b562, 3-chloro-N-[trans-4-(methylamino)cyclohexyl]-N-{[3-(pyridin-4-yl)phenyl]methyl}-1-benzothiophene-2-carboxamide, CHOLESTEROL, ... (6 entities in total)
Functional Keywords	class f g protein coupled receptor, hedgehog signalling, oncoprotein, drug target, signal transduction, morphogen, cholesterol, membrane protein
Biological source	Homo sapiens (human) More
Total number of polymer chains	2
Total formula weight	143945.09
Authors	Byrne, E.F.X.,Woolley, R.E.,Ansell, B.,Sansom, M.S.P.,Newstead, S.,Siebold, C. (deposition date: 2022-04-07, release date: 2022-06-15, Last modification date: 2024-01-31)
Primary citation	Kinnebrew, M.,Woolley, R.E.,Ansell, T.B.,Byrne, E.F.X.,Frigui, S.,Luchetti, G.,Sircar, R.,Nachtergaele, S.,Mydock-McGrane, L.,Krishnan, K.,Newstead, S.,Sansom, M.S.P.,Covey, D.F.,Siebold, C.,Rohatgi, R. Patched 1 regulates Smoothened by controlling sterol binding to its extracellular cysteine-rich domain. Sci Adv, 8:eabm5563-eabm5563, 2022 Cited by PubMed: 35658032 DOI: 10.1126/sciadv.abm5563 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3 Å)

Structure validation