7ZHR

Complex structure of drosophila Unr CSD789 and pAbp RRM3

7ZHR の概要

• エントリーDOI: 10.2210/pdb7zhr/pdb
• 分子名称: Upstream of N-ras, isoform A, Polyadenylate-binding protein (3 entities in total)
• 機能のキーワード: drosophila unr, csd, cold-shock domain, protein complex, ppi, drosophila pabp, rrm, unr, pabp, rna binding protein
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36114.56

構造登録者

Hollmann, N.M.,Jagtap, P.K.A.,Hennig, J. (登録日: 2022-04-07, 公開日: 2022-12-07, 最終更新日: 2024-02-07)

主引用文献

Hollmann, N.M.,Jagtap, P.K.A.,Linse, J.B.,Ullmann, P.,Payr, M.,Murciano, B.,Simon, B.,Hub, J.S.,Hennig, J.
Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein.
Nucleic Acids Res., 51:1895-1913, 2023

PubMed Abstract: RNA binding proteins (RBPs) often engage multiple RNA binding domains (RBDs) to increase target specificity and affinity. However, the complexity of target recognition of multiple RBDs remains largely unexplored. Here we use Upstream of N-Ras (Unr), a multidomain RBP, to demonstrate how multiple RBDs orchestrate target specificity. A crystal structure of the three C-terminal RNA binding cold-shock domains (CSD) of Unr bound to a poly(A) sequence exemplifies how recognition goes beyond the classical ππ-stacking in CSDs. Further structural studies reveal several interaction surfaces between the N-terminal and C-terminal part of Unr with the poly(A)-binding protein (pAbp). All interactions are validated by mutational analyses and the high-resolution structures presented here will guide further studies to understand how both proteins act together in cellular processes.

PubMed: 36688322
DOI: 10.1093/nar/gkac1277

実験手法

X-RAY DIFFRACTION (2.99 Å)