7ZHK

GPN-loop GTPase from Sulfolobus acidocaldarius open state (GDP)

7ZHK の概要

• エントリーDOI: 10.2210/pdb7zhk/pdb
• 関連するPDBエントリー: 7ZHF
• 分子名称: GTPase, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: gpn-loop, self-activating, gtpase, homodimer, unknown function
• 由来する生物種: Sulfolobus acidocaldarius
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 93982.01

構造登録者

Korf, L.,Essen, L.-O. (登録日: 2022-04-06, 公開日: 2023-10-25, 最終更新日: 2024-05-08)

主引用文献

Korf, L.,Ye, X.,Vogt, M.S.,Steinchen, W.,Watad, M.,van der Does, C.,Tourte, M.,Sivabalasarma, S.,Albers, S.-V.,Essen, L.-O.
Archaeal GPN-loop GTPases involve a lock-switch-rock mechanism for GTP hydrolysis.
Mbio, 14:e0085923-e0085923, 2023

PubMed Abstract: GPN-loop GTPases have been found to be crucial for eukaryotic RNA polymerase II assembly and nuclear trafficking. Despite their ubiquitous occurrence in eukaryotes and archaea, the mechanism by which these GTPases mediate their function is unknown. Our study on an archaeal representative from showed that these dimeric GTPases undergo large-scale conformational changes upon GTP hydrolysis, which can be summarized as a lock-switch-rock mechanism. The observed requirement of GPN for motility appears to be due to its large footprint on the archaeal proteome.

PubMed: 37962382
DOI: 10.1128/mbio.00859-23

実験手法

X-RAY DIFFRACTION (2.4 Å)