7ZGW

Serratia NucC apo form

7ZGW の概要

• エントリーDOI: 10.2210/pdb7zgw/pdb
• 分子名称: Serratia NucC (2 entities in total)
• 機能のキーワード: nuclease, rna binding protein
• 由来する生物種: Serratia
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 173380.22

構造登録者

Garcia-Doval, C.,Mayo-Munoz, D.,Smith, L.M.,Fineran, P.C. (登録日: 2022-04-04, 公開日: 2022-10-26, 最終更新日: 2024-02-07)

主引用文献

Mayo-Munoz, D.,Smith, L.M.,Garcia-Doval, C.,Malone, L.M.,Harding, K.R.,Jackson, S.A.,Hampton, H.G.,Fagerlund, R.D.,Gumy, L.F.,Fineran, P.C.
Type III CRISPR-Cas provides resistance against nucleus-forming jumbo phages via abortive infection.
Mol.Cell, 82:4471-4486.e9, 2022

PubMed Abstract: Bacteria have diverse defenses against phages. In response, jumbo phages evade multiple DNA-targeting defenses by protecting their DNA inside a nucleus-like structure. We previously demonstrated that RNA-targeting type III CRISPR-Cas systems provide jumbo phage immunity by recognizing viral mRNA exported from the nucleus for translation. Here, we demonstrate that recognition of phage mRNA by the type III system activates a cyclic triadenylate-dependent accessory nuclease, NucC. Although unable to access phage DNA in the nucleus, NucC degrades the bacterial chromosome, triggers cell death, and disrupts phage replication and maturation. Hence, type-III-mediated jumbo phage immunity occurs via abortive infection, with suppression of the viral epidemic protecting the population. We further show that type III systems targeting jumbo phages have diverse accessory nucleases, including RNases that provide immunity. Our study demonstrates how type III CRISPR-Cas systems overcome the inaccessibility of jumbo phage DNA to provide robust immunity.

PubMed: 36395770
DOI: 10.1016/j.molcel.2022.10.028

実験手法

X-RAY DIFFRACTION (1.83 Å)