7ZDU
Occ(apo/return) conformation of CydDC mutant (E500Q.C) in ATP(CydC)/ATP(CydD) bound state (Dataset-19)
Summary for 7ZDU
| Entry DOI | 10.2210/pdb7zdu/pdb |
| Related | 7ZD5 |
| EMDB information | 14636 14670 |
| Descriptor | ATP-binding/permease protein CydC, ATP-binding/permease protein CydD, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | abc transporter, cyddc, heme transporter, membrane protein |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 2 |
| Total formula weight | 129161.71 |
| Authors | Wu, D.,Safarian, S. (deposition date: 2022-03-29, release date: 2023-04-19, Last modification date: 2023-11-01) |
| Primary citation | Wu, D.,Mehdipour, A.R.,Finke, F.,Goojani, H.G.,Groh, R.R.,Grund, T.N.,Reichhart, T.M.B.,Zimmermann, R.,Welsch, S.,Bald, D.,Shepherd, M.,Hummer, G.,Safarian, S. Dissecting the conformational complexity and mechanism of a bacterial heme transporter. Nat.Chem.Biol., 19:992-1003, 2023 Cited by PubMed Abstract: Iron-bound cyclic tetrapyrroles (hemes) are redox-active cofactors in bioenergetic enzymes. However, the mechanisms of heme transport and insertion into respiratory chain complexes remain unclear. Here, we used cellular, biochemical, structural and computational methods to characterize the structure and function of the heterodimeric bacterial ABC transporter CydDC. We provide multi-level evidence that CydDC is a heme transporter required for functional maturation of cytochrome bd, a pharmaceutically relevant drug target. Our systematic single-particle cryogenic-electron microscopy approach combined with atomistic molecular dynamics simulations provides detailed insight into the conformational landscape of CydDC during substrate binding and occlusion. Our simulations reveal that heme binds laterally from the membrane space to the transmembrane region of CydDC, enabled by a highly asymmetrical inward-facing CydDC conformation. During the binding process, heme propionates interact with positively charged residues on the surface and later in the substrate-binding pocket of the transporter, causing the heme orientation to rotate 180°. PubMed: 37095238DOI: 10.1038/s41589-023-01314-5 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.98 Å) |
Structure validation
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