7ZD2

Crystal structure of Pseudomonas aeruginosa S-adenosyl-L-homocysteine hydrolase inhibited by Co2+ ions.

7ZD2 の概要

• エントリーDOI: 10.2210/pdb7zd2/pdb
• 分子名称: Adenosylhomocysteinase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ADENOSINE, ... (10 entities in total)
• 機能のキーワード: regulation of sam-dependent methylation reactions, inhibition, metal binding, cobalt, hydrolase
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 212383.50

構造登録者

Malecki, P.H.,Gawel, M.,Brzezinski, K. (登録日: 2022-03-29, 公開日: 2023-04-19, 最終更新日: 2024-10-30)

主引用文献

Gawel, M.,Malecki, P.H.,Sliwiak, J.,Stepniewska, M.,Imiolczyk, B.,Czyrko-Horczak, J.,Jakubczyk, D.,Marczak, L.,Plonska-Brzezinska, M.E.,Brzezinski, K.
A closer look at molecular mechanisms underlying inhibition of S -adenosyl-L-homocysteine hydrolase by transition metal cations.
Chem.Commun.(Camb.), 60:11504-11507, 2024

PubMed Abstract: We report biochemical and structural studies on inhibiting bacterial -adenosyl-L-homocysteine hydrolase by transition metal cations. Our results revealed diverse molecular mechanisms of enzyme inactivation. Depending on the cation, the mechanism is based on arresting the enzyme in its closed, inactive conformation, disulfide bond formation within the active site or oxidation of the intermediate form of a cofactor.

PubMed: 39230573
DOI: 10.1039/d4cc03143a

実験手法

X-RAY DIFFRACTION (2.16 Å)