7ZCG

CHMP2A-CHMP3 heterodimer (430 Angstrom diameter)

7ZCG の概要

• エントリーDOI: 10.2210/pdb7zcg/pdb
• EMDBエントリー: 14630
• 分子名称: Charged multivesicular body protein 2a, Charged multivesicular body protein 3 (2 entities in total)
• 機能のキーワード: cryo electron microscopy, helical reconstruction, membrane-bound chmp2a-chmp3 filament, negative-curvature membrane, cytosolic protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35735.17

構造登録者

Azad, K.,Desfosses, A.,Effantin, G.,Schoehn, G.,Weissenhorn, W. (登録日: 2022-03-28, 公開日: 2023-01-18, 最終更新日: 2024-07-24)

主引用文献

Azad, K.,Guilligay, D.,Boscheron, C.,Maity, S.,De Franceschi, N.,Sulbaran, G.,Effantin, G.,Wang, H.,Kleman, J.P.,Bassereau, P.,Schoehn, G.,Roos, W.H.,Desfosses, A.,Weissenhorn, W.
Structural basis of CHMP2A-CHMP3 ESCRT-III polymer assembly and membrane cleavage.
Nat.Struct.Mol.Biol., 30:81-90, 2023

PubMed Abstract: The endosomal sorting complex required for transport (ESCRT) is a highly conserved protein machinery that drives a divers set of physiological and pathological membrane remodeling processes. However, the structural basis of ESCRT-III polymers stabilizing, constricting and cleaving negatively curved membranes is yet unknown. Here we present cryo-EM structures of membrane-coated CHMP2A-CHMP3 filaments from Homo sapiens of two different diameters at 3.3 and 3.6 Å resolution. The structures reveal helical filaments assembled by CHMP2A-CHMP3 heterodimers in the open ESCRT-III conformation, which generates a partially positive charged membrane interaction surface, positions short N-terminal motifs for membrane interaction and the C-terminal VPS4 target sequence toward the tube interior. Inter-filament interactions are electrostatic, which may facilitate filament sliding upon VPS4-mediated polymer remodeling. Fluorescence microscopy as well as high-speed atomic force microscopy imaging corroborate that VPS4 can constrict and cleave CHMP2A-CHMP3 membrane tubes. We therefore conclude that CHMP2A-CHMP3-VPS4 act as a minimal membrane fission machinery.

PubMed: 36604498
DOI: 10.1038/s41594-022-00867-8

実験手法

ELECTRON MICROSCOPY (3.3 Å)