7ZC6

Na+ - translocating ferredoxin: NAD+ reductase (Rnf) of C. tetanomorphum

7ZC6 の概要

• エントリーDOI: 10.2210/pdb7zc6/pdb
• EMDBエントリー: 14622
• 分子名称: RnfA, RIBOFLAVIN, RnfB, ... (10 entities in total)
• 機能のキーワード: rnf, flavin, iron-sulfur cluster, sodium ion translocation, anaerobic energy metabolism, oxidoreductase, electron transport, redox-coupled sodium pump, flavoprotein
• 由来する生物種: Clostridium tetanomorphum; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 173737.84

構造登録者

Ermler, U.,Vitt, S.,Buckel, W. (登録日: 2022-03-25, 公開日: 2022-09-28, 最終更新日: 2024-10-23)

主引用文献

Vitt, S.,Prinz, S.,Eisinger, M.,Ermler, U.,Buckel, W.
Purification and structural characterization of the Na + -translocating ferredoxin: NAD + reductase (Rnf) complex of Clostridium tetanomorphum.
Nat Commun, 13:6315-6315, 2022

PubMed Abstract: Various microbial metabolisms use H/Na-translocating ferredoxin:NAD reductase (Rnf) either to exergonically oxidize reduced ferredoxin by NAD for generating a transmembrane electrochemical potential or reversely to exploit the latter for producing reduced ferredoxin. For cryo-EM structural analysis, we elaborated a quick four-step purification protocol for the Rnf complex from Clostridium tetanomorphum and integrated the homogeneous and active enzyme into a nanodisc. The obtained 4.27 Å density map largely allows chain tracing and redox cofactor identification complemented by biochemical data from entire Rnf and single subunits RnfB, RnfC and RnfG. On this basis, we postulated an electron transfer route between ferredoxin and NAD via eight [4Fe-4S] clusters, one Fe ion and four flavins crossing the cell membrane twice related to the pathway of NADH:ubiquinone reductase. Redox-coupled Na translocation is provided by orchestrating Na uptake/release, electrostatic effects of the assumed membrane-integrated FMN semiquinone anion and accompanied polypeptide rearrangements mediated by different redox steps.

PubMed: 36274063
DOI: 10.1038/s41467-022-34007-z

実験手法

ELECTRON MICROSCOPY (4.27 Å)